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Table 4 Fine mapping of qGS1-35.2 using five sets of near isogenic lines in BC2F14:15 and BC2F15:16

From: Dissection and fine-mapping of two QTL for grain size linked in a 460-kb region on chromosome 1 of rice

Generation

Name

Traita

Phenotype (mean ± sd)b

P

A c

R2(%)d

NILZS97

NILMY46

BC2F14:15

Q1

TGW

27.66 ± 0.20

27.61 ± 0.22

0.3588

  
 

GL

8.032 ± 0.033

7.989 ± 0.034

< 0.0001

−0.022

26.1

 

GW

3.235 ± 0.015

3.242 ± 0.015

0.0917

  
 

RLW

2.483 ± 0.014

2.464 ± 0.010

< 0.0001

−0.010

18.2

Q2

TGW

27.57 ± 0.26

27.60 ± 0.26

0.7044

  
 

GL

8.023 ± 0.034

7.964 ± 0.027

< 0.0001

−0.029

33.4

 

GW

3.222 ± 0.014

3.235 ± 0.018

0.0084

0.006

7.5

 

RLW

2.490 ± 0.011

2.462 ± 0.014

< 0.0001

−0.014

34.3

Q3

TGW

28.00 ± 0.34

28.13 ± 0.28

0.0986

  
 

GL

8.015 ± 0.036

8.017 ± 0.026

0.8229

  
 

GW

3.246 ± 0.017

3.249 ± 0.015

0.4148

  
 

RLW

2.470 ± 0.014

2.468 ± 0.014

0.5968

  

BC2F15:16

B1

TGW

28.80 ± 0.27

28.76 ± 0.29

0.6390

  
 

GL

8.386 ± 0.030

8.325 ± 0.036

< 0.0001

−0.030

29.0

 

GW

3.128 ± 0.017

3.141 ± 0.021

0.0070

0.006

5.2

 

GT

2.193 ± 0.014

2.197 ± 0.009

0.1490

  
 

RLW

2.681 ± 0.011

2.651 ± 0.018

< 0.0001

−0.015

36.5

 

NP

16.54 ± 1.79

16.44 ± 2.19

0.8313

  
 

NGP

55.60 ± 5.84

57.12 ± 8.90

0.3960

  
 

GY

24.28 ± 1.28

24.67 ± 1.88

0.3065

  

B2

TGW

27.94 ± 0.44

28.04 ± 0.30

0.2894

  
 

GL

8.438 ± 0.047

8.443 ± 0.041

0.6192

  
 

GW

3.055 ± 0.027

3.057 ± 0.019

0.6095

  
 

GT

2.204 ± 0.011

2.209 ± 0.011

0.0843

  
 

RLW

2.762 ± 0.021

2.760 ± 0.014

0.7448

  
 

NP

15.16 ± 0.93

15.42 ± 1.13

0.3456

  
 

NGP

69.04 ± 7.68

67.82 ± 6.95

0.5098

  
 

GY

24.12 ± 3.38

24.11 ± 3.44

0.9599

  
  1. aTGW 1000-grain weight (g), GL grain length (mm), GW grain width (mm), GT grain thickness (mm), RLW ratio of grain length to width, NP number of panicles per plant, NGP number of grains per panicle, GY grain yield per plant (g)
  2. bNILZS97 and NILMY46 are near isogenic lines with Zhenshan 97 and Milyang 46 homozygous genotypes in the segregating region, respectively
  3. cAdditive effect of replacing a Zhenshan 97 allele with a Milyang 46 allele
  4. dProprotion of phenotypic variance explained by the QTL effect