Fig. 1

Circadian clock-controlled OsRACK1A expression in rice. a Transcript analysis of the OsRACK1A gene by qRT-PCR over a 48-h period. Rice plants were grown under 16 h light and 8 h dark in a growth chamber at a constant temperature of 28 °C and sampled at regular intervals. b Immunoblot analysis of OsRACK1A protein over a 24-h period. c OsRACK1A expression was monitored by immunoblot analysis in seedlings of a non-transgenic line (NTL), an OsRACK1A-overexpressing transgenic line (OeTL3–8), and an RNA-interfered transgenic line (RiTLs4–2). d Protein levels of OsRACK1A in NTL, OeTL3–8 and RiTLs4–2 in light and dark. e Heading time distributions in transgenic rice plants grown in the field. During the heading period, the number of heading plants for each line was recorded every 3 days and compared. f Phenotypic comparison panicles during the heading period of transgenic plants. The rice plants had been grown in the field for 89 d. ZT, Zeitgeber Time. White and black rectangles indicate lights on and lights off, respectively