Expression Profile of Defense Genes in Rice Lines Pyramided with Resistance Genes Against Bacterial Blight, Fungal Blast and Insect Gall Midge

Divya, Dhanasekar; Madhavi, Kanaparthi Ratna; Dass, Muralidharan Ayyappa; Maku, Roshan Venkata; Mallikarjuna, Garladinne; Sundaram, Raman Meenakshi; Laha, Gouri Sankar; Padmakumari, Ayyagari Phani; Patel, Hitendra Kumar; Prasad, Madamsetty Srinivas; Sonti, Ramesh Venkata; Bentur, Jagadish Sanmallappa

doi:10.1186/s12284-018-0231-4

Rice

Table 2 Selected defense related genes used in validation studies and cDNA based primers

From: Expression Profile of Defense Genes in Rice Lines Pyramided with Resistance Genes Against Bacterial Blight, Fungal Blast and Insect Gall Midge

S. No.	FC^a	Locus ID	Identity/Function	cDNA based primer sequences	Reference
1	22.71	LOC_Os10g37160	Cytochrome p450 family/induced upon defense response	F:GTTCTGCCTCCTCGTGAATA R:GGCTCGTGATGTAGATGAGC	^a
2	18.72	LOC_Os08g07080	Terpene synthase 10, putative/secondary metabolism, volatile metabolites	F:GGCTCGAGTGAAGTACCAGA R:CAATGCAGAGAAGGTCGTTT	^a
3	13.37	LOC_Os01g03680	Bowman-birk trypsin inhibitor/inhibits insect proteolytic enzymes	F:GACAAGGTGAAGTCGTGCTC R:TTAAGCTGGCTGGTTGTGAC	^a
4	10.13	LOC_Os12g37260	Lipoxygenase 2.1, chloroplast/involved in JA biosynthesis	F:TGGAGCTGACGATAGAGGAC R:CACATAATCCGACACCCACT	^a
5	4.87	LOC_Os01g65110	POT family protein, expressed/Induced in BL infected plants with Pi54	F:GTCGCCTTCTTCCTCTTCTC R:CAGATGCCATCATCATCAAC	^a, Gupta et al. 2011
6	4.71	LOC_Os07g03710	SCP-like extracellular protein/PR1, induced by Mo and Xoo infection (ref.)	F:GAAGTACGGCGAGAACATCT R:GTGGTCGTACCACTGCTTCT	^a
7	3.05	LOC_Os01g71340	Glucan endo-1,3-beta-glucosidase/PR2 Induced by fungal infection	F:GCAGACGTACAACCAGAACC R:GAACATGGCGAAAATGTAGG	^a, Balasubramanian et al. 2012
8	–	LOC_Os12g36830	PR 10a/involved in Gm11 mediated resistance	F:ACCATCTACACCATGAAGCTTAAC R:GTATTCCTCTTCATCTTAGGCGTA	Rawat et al. 2013 Pruitt et al. 2015
9	–	LOC_Os10g01660	Isoflavone reductase	F:AGAAGAAGACGGGGAAGAAG R:GATCTCCGACTCCTGGATTT	Peng et al. 2015
10	–	LOC_Os11g42010 (AY914077)	Pi54/one of the pyramided genes	F:AAGATTTTCGAGGCTCTTCTCTA R:GATGAATCTGTTTCCTCGTCTTG	Rai et al. 2011
11	–	LOC_Os08g09670.1	Gm4/one of pyramided genes	F: CGCTTCAGACTGAGTCAACA R: CTTCCAATCCTTCATTGGTG	Divya et al. 2015
12	–	LOC_Os04g52970	gm3 – one of the pyramided genes	F:TCTGGCCTGCACGAAGC R:GGCAAACGCCTACCCAGGA	Sama et al. 2014
13	–	LOC_Os08g15080	Gm8 – one of the pyramided genes	F:ATCGCCGCCAAGGCCGCGCT R:ATGATATGGGGGAGCAGCAT	Divya, 2016
14	–	LOC_Os11g45990	von Willebrand factor type A protein Involved in Gm1 mediated resistance	F:AGTTTGTCATCAGGAAGCTTGCT R:GCTATATTCCTTGACGGGTCCAT	Rawat et al. 2012

^aDesigned for this study; − Not tested

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