Fig. 5

The stomatal aperture status in the osmdhar4 mutant and OsMDHAR4-overexpressing plants. a Scanning electron microscopy images of three levels of stomatal opening. Scale bars = 2 μm. b Percentages of three levels of stomatal opening in WT and the osmdhar4 mutant under normal conditions (48 stomata for WT, 40 stomata for the osmdhar4 mutant) and heat stress conditions (28 stomata for WT, 25 stomata for the osmdhar4 mutant). c Stomatal conductance of WT and osmdhar4 mutant under normal conditions and heat stress conditions (3 repeats, 12 plants in each repeat). d Percentages of three levels of stomatal opening in WT and OsMDHAR4-OE-1 plants under normal conditions (70 stomata for WT, 90 stomata for OsMDHAR4-OE-1) and heat stress conditions (60 stomata for WT, 76 stomata for OsMDHAR4-OE-1). e Stomatal conductance of the WT and OsMDHAR4-OE-1 under normal conditions and heat stress conditions (3 repeats, 12 plants in each repeat). FW, Fresh Weight. Error bars indicate the SE based on three biological replicates. *, P < 0.05, and **, P < 0.01, by Student’s t-test