Skip to main content
Rice
Fig. 5 | Rice

Fig. 5

From: OsSND2, a NAC family transcription factor, is involved in secondary cell wall biosynthesis through regulating MYBs expression in rice

Fig. 5

Generation and analysis of snd2 mutants. a Schematic diagram of OsSND2 gene structure and two CRISPR/Cas9 target sites. UTRs, exons, and introns are indicated by blank rectangles, black rectangles, and black lines, respectively. b DNA sequence alignments for the two homozygous snd2 mutants identified in the T1 generation, together with a wild-type (WT) control. The numbers on the right side are the sizes of the indels, with “−” and “+” showing deletion and insertion of nucleotides involved, respectively. c Deduced OsSND2 amino acid sequence alignments for the two homozygous mutants and WT. d Three-month-old plant of wild type (WT) and snd2-c1 mutant. Bar = 10 cm. e Measurement of cellulose content in WT and snd2 mutants. f Relative expression of OsMYB61 and SCWs-related CESA genes in WT and snd2 mutants. The Actin1 gene was used as internal control. Error bars, SD of three biological replicates. g Observation of sclerenchyma cell walls in the internodes from the three-month-old wild-type and snd2 mutant plants via transmission electron microscope. Bar = 2 μm

Back to article page