Fig. 6

qPCR and amino acid analysis in the basal portions of wild-type and as1 mutant shoots. qPCR analysis of OsAS2 (a) and Actin1 (b) and analyses of asparagine (c), glutamine (d) and NH₄⁺ contents (e) were performed in the basal portions of shoots of the wild type (WT: black column) and two lines of as1 mutants (as1-m1 and as1-m2: dark and light grey column, respectively). Seedlings were grown hydroponically in the presence of 1 mM NH₄Cl until the fourth-leaf stage. Mean values with SE of four independent samples are shown. One-way ANOVAs followed by Bonferroni tests were used to identify significant differences between the wild type and as1 mutants (P < 0.05)