A Versatile Vector Toolkit for Functional Analysis of Rice Genes

Rice

Table 1 The cloning compatibility of the synthesized MCS for rice CDSs

CDSs	Number	Percentage	Total coverage
Containing 5 cut sites	1403	2.51
Containing 6 cut sites	245	0.44
Containing 7 cut sites	7	0.01
Others (< 5 cut sites)	54,146	97.03
Total	55,801	100	99.55%

Rice CDSs refer to the MSU RGAP database Version7 (http://rice.plantbiology.msu.edu/). Compatible genes should contain ≤5 restriction enzyme recognition sites of the MCS in double digestion-ligation cloning