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Table 1 Primers used in this study

From: Identification of resistant germplasm containing novel resistance genes at or tightly linked to the Pi2/9 locus conferring broad-spectrum resistance against rice blast

Primer name

Primer sequence (5′ to 3′)

Purpose

Expected Size (bp)

Pi2/9-DF1

CTTGACATCCAAACCGCACC

For the development of the marker Pi2/9-RH

1172

Pi2/9-DR1

TAGGCCTAGCCAATTTTTGCC

Pi2/9-DF2

CAGACGCTGCCGAAGGCTGC

For the development of the marker Pi2/9-RH

1512

Pi2/9-DR2

CAATAGTTGCTGATTCCTGAGC

5UTR-F

CTTGAAGGGAGAGTCGAACG

For the cloning of full-length cDNA of Pi2/9 homologues

3235

3UTR-R

GCCTCATTGATCATCATGCC