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Fig. 2 | Rice

Fig. 2

From: Overexpression of Rice Auxilin-Like Protein, XB21, Induces Necrotic Lesions, up-Regulates Endocytosis-Related Genes, and Confers Enhanced Resistance to Xanthomonas oryzae pv. oryzae

Fig. 2

Rice XB21 interacts with XA21 in vivo. XB21 co-immunoprecipitated with XA21 protein before (Mock) and after Xoo inoculation (Xoo) in Kitaake (Kit) and transgenic rice carrying Myc-XA21 under the control of its native promoter (Myc-XA21, T330-16-1). The precipitates were used for western gel blot analysis using anti-Myc antibody (a) or anti-XB21 antibody (b). Myc-XA21 and its cleavage product displayed bands at approximately 140 and 100 kDa, respectively, and XB21 was detected as approximately 110 kDa band. Experimental results were repeated three times, with similar results

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