Fig. 4

Binding models of selected ligands in the carbohydrate-binding site of OsEULS2 and OsEULD1A. A-C. Side-view of the carbohydrate-binding site of OsEULS2 showing the docking of Man1,2Man (a), Man (b) or LacNAc (c) to the carbohydrate-binding site. d-i. Side-view of the carbohydrate-binding site of OsEULD1A showing the docking of Man1,2Man (d-e), Man (f-g) or LacNAc (h-i) to the carbohydrate-binding site of the first (d, f and h) or second (e, g and i) EUL domain. The H-bond distances are indicated (Å). A stacking interaction occurs between the first Man ring (a, d and e), Man (b, f and g) or Gal (c, h and i) and the aromatic residues F118 and W136 (colored orange) located in the vicinity of the active site