Fig. 2

Identification of binding motifs of recombinant OsSMF1 from E. coli by Q9-PBM. a, c, e The consensus binding sequences according to the binding intensities observed on the Q9 protein-binding microarray. The overall consensus binding motif was obtained by multiple alignments of the significant binding sequences with ClustalW, and it was visualized with SeqLogo program. b, d, f The effects of mutations on the binding intensities of the consensus binding motif. The average binding intensities (+) of probes containing TGAGTCA (b), CCACGTC (d), and GGATGAC (f) were plotted. The yellow boxes represent the average binding intensities of probes substituted at each position with other bases