Fig. 4

Overexpression of PsAKR1 in susceptible rice genotype Tellahamsa showed improved seed viability and germination. a Transcript levels of PsAKR1 in different transgenic rice lines, The RNA from seeds were isolated and the cDNA is used as a template, b Photograph of transgenic rice seeds showing early germination after ageing treatment, c Germination percentage of transgenic seeds under 0 day and 6 days of AA is more compared to wild type Tellahamsa seeds, d AKR1 enzyme activity in rice seeds under control and ageing conditions. The total protein was extracted and enzyme activity was determined against Methylglyoxal as substrate. Minimum of three biological replicates were used in all the assays