Fig. 3

Suppression of OsVPE3 improved survival rate and inhibited chlorosis under salt stress. a Plants were grown for 4 weeks, treated with 150 mMNaCl for 3 days and then allowed to recover for 7 days. b Expression levels of OsVPE3 in WT and transgenic lines as determined by qRT-PCR. Total RNA was extracted from the fourth leaves. OsUBQ5 was used as an internal control. Values are means, and error bars represent the SD from three independent experiments. Asterisks indicate a significant difference between WT and transgenic lines (t-test; **, P < 0.01). c The fourth leaves of NaCl-treated plants. Bar = 1 cm. d Chlorophyll retention in the fourth leaves of NaCl-treated plants prior to recovery. Values are means, and error bars represent the SD from three independent experiments. Asterisks indicate a significant difference between WT and transgenic lines (t-test; *, P < 0.05). e Survival rates of NaCl-treated plants 7 days after recovery. Values are means, and error bars represent the SD (n > 20) of three independent experiments. Asterisks indicate a significant difference between WT and transgenic lines (t-test; *, P < 0.05)