Fig. 2

Generation and analysis of transgenic lines. A diagram of the vector constructs used to generate the overexpression lines (a) and RNAi line (b). c Expression levels of OsVPE3 in WT and transgenic lines were determined by qRT-PCR. OsUBQ5 was used as an internal control. Values are means, and error bars represent the SD (n > 1) from three independent experiments. Asterisks indicate a significant difference between WT and transgenic lines (t-test; **, P < 0.01). d SDS-PAGE analysis of seed storage proteins in WT and transgenic lines. The intensity of protein bands was measured by Image J software. Arrow indicates the 57-kDa glutelin precursor