Fig. 4
From: SMALL GRAIN 11 Controls Grain Size, Grain Number and Grain Yield in Rice
Identification of the D2/SMG11 gene. a The D2/SMG11 gene structure. The white boxes indicate the exons, and black lines indicate the introns. The start codon (ATG) and the stop codon (TAG) are indicated. The smg11 mutation in the SMG11/D2 gene is shown. b The dCAPS marker is developed according to the smg11 mutation. c The D2/SMG11 protein contains a membrane anchor domain (N1), a proline-rich domain (N2), a dioxygen binding domain (N3), a steroid binding domain (N4) and a heme binding domain (N5). d e Grains of KYJ, smg11, pActin: SMG11;smg11#1 and pActin: SMG11;smg11#2. pActin: SMG11;smg11 is smg11 transformed with pActin: SMG11. f g h Grain length f grain width g and 1000-grain weight h of KYJ, smg11, pActin: SMG11;smg11#1 and pActin: SMG11;smg11#2. i The number of KYJ, smg11, pActin: SMG11;smg11#1 and pActin: SMG11;smg11#2 secondary panicle branches. j Grain number per panicle of KYJ, smg11, pActin: SMG11;smg11#1 and pActin: SMG11;smg11#2. k Quantitative real-time RT-PCR analysis of SMG11/D2 gene expression in developing panicles of 1 cm (YP1) to 20 cm (YP20). Values f–k are given as mean ± SD. *P < 0.05; **P < 0.01 compared with smg11 by Student’s t-test. Bars: 2 mm in d-e