Fig. 3
From: The Rice Pentatricopeptide Repeat Gene TCD10 is Needed for Chloroplast Development under Cold Stress
Genetic analysis and cloning of the TCD10 gene; a TCD10 was located between the RM25441 and RM333 on chromosome 10 using 222 F2 mutant individuals; b TCD10 was narrowed to a 70 kb between ID14874 and ID14944 on AC027662 BAC clone using 5200 F2 mutant individuals; c The target region contains six predicted candidate genes (LOC_Os10g28590, LOC_Os10g28600 LOC_Os10g28610, LOC_Os10g28620, LOC_Os10g28630, LOC_Os10g28640); d A single nucleotide (A) deletion mutation at position 766 bp from the ATG start codon in LOC_Os10g28600; e Chloroplast transit peptide (CTP), PPR motif and mutation sites; f Phenotypes of CRISPR/Cas9 genome editing (T1-1 and T1-2) and RNAi (T1-3) transgenic lines grown at 20 °C; g Transcript levels of three RNAi (T1-3) transgenic lines