Fig. 9
From: The existence of C4-bundle-sheath-like photosynthesis in the mid-vein of C3 rice
Immunoblot analysis of photosynthetic subunits in rice mid-veins and leaf laminae. a For protein loading (15 μg per spot) verification, SDS–PAGE gels were stained with Coomassie brilliant blue R-250. Pre-stained protein marker was indicated on the left, and bands corresponding to PsaA, large subunit of RuBisCO, PsbA, PsbO, LHCII, PsbP, and small subunit of RuBisCO/PsbQ were shown on the right. b Immunoblot analysis was carried out using the polyclonal antibodies against the thylakoid membrane subunits. Quantitation of band signal was performed using Quantity One (Bio-Rad, Hercules, USA) based on densitometric analysis. The amount of protein was expressed as the percentage of that in leaf laminae (100 %). Data are means ± SE from three independent experiments, with the asterisk indicating statistically significant differences (P < 0.05) between mid-veins and leaf laminae