Fig. 1
From: The existence of C4-bundle-sheath-like photosynthesis in the mid-vein of C3 rice
Phenotypic characteristics of rice mid-veins and leaf laminae. a The photograph of the midsection of a rice leaf, scale bar = 3 cm. b The optical micrograph of transverse sections of a rice leaf, and green areas illustrate the distribution of chlorophyllous cells. c The epifluorescence micrograph of transverse sections of a rice leaf, and chlorophyllous cells can be identified by the bright red fluorescence, scale bar =250 μm. Transverse sections of leaves were subjected to the epifluorescence microscopy. However, because of the intrinsic background noise from the epifluorescence microscopy and the low resolution of the Insight camera (CFI60, Nikon), images had to be first treated with Photoshop (CS5, Adobe) to discard the noise, which may have affected their quality (Wittmann et al. 2006; Berveiller and Damesin 2008)