Fig. 2

A CAPS marker targeting the LRR domain of Pi2. a DNA polymorphisms corresponding to the eight amino-acid differences in between Pi2 and Piz-t. b Sequence alignment showing that the polymorphic variations corresponding to the first six amino-acid differences in between Pi2 and Piz-t were conserved between the Pi2 and non-Pi2 alleles in diverse cultivars. c Schematic diagrams indicating sizes and recognition sites for HinfI and PstI in the PCR fragment of the Pi2 and Piz-t alleles amplified with primers 2-LRR-F/2-LRR-R. d Electrophoresis profiles of the CAPS marker Pi2-LRR that differentiates the Pi2 and non-Pi2 alleles. Upper panel showing the PCR products without digestion with restriction enzymes, middle panel showing the PCR products digested with PstI, and lower panel showing the PCR products digested with HinfI. M: DNA ladder; 1: C101A51 (Pi2 donor line); 2: Toride-1 (Piz-t donor line); 3: 75-1-127 (Pi9 donor line); 4: Nipponbare; 5: 93–11; 6: CO39; 7: D62B; 8: Xianghui 68; 9: Shuhui 527; 10: Zhenshan 97B; 11: Zaogang B; 12: Minghui 63; 13: Guangzhan 63S; 14: Gang 46B; 15: Miyang 46; 16: Fu 838; 17: SE21S; 18: Peiai 64S; 19: Lijiangxintuanheigu; 20: Minhui 3301; 21: Longtepu B; 22: 02428; 23: Guanghui 998; 24: Jin 23B. PstI recognition sequence (CTGCAG) is underlined; HinfI recognition sequence (GAATC) is marked by dotted underling