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Fig. 1 | Rice

Fig. 1

From: Allele-specific marker-based assessment revealed that the rice blast resistance genes Pi2 and Pi9 have not been widely deployed in Chinese indica rice cultivars

Fig. 1

An InDel marker targeting the promoter region of Pi9. a Physical location of the identified InDel region in the Pi2/9 locus. b Sequence alignment showing the 27-bp InDel between the Pi2/Piz-t and non-Pi2/Piz-t alleles and the 10-bp InDel between the Pi9 and non-Pi9 alleles in diverse cultivars. c PCR amplification patterns of the InDel marker Pi9-Pro that differentiates the Pi2/Piz-t, Pi9 and non-Pi2/Piz-t/Pi9 alleles. M: DNA ladder; 1: C101A51 (Pi2 donor line); 2: Toride-1 (Piz-t donor line); 3: 75-1-127 (Pi9 donor line); 4: Nipponbare; 5: 93–11; 6: CO39; 7: D62B; 8: SE21S; 9: Katy; 10: 02428; 11: Longtepu B; 12: Lijiangxintuanheigu; 13: Shuhui 527; 14: Minhui 3301; 15: Ce 64; 16: Fu 838; 17: Guanghui 998; 18: Miyang 46; 19: Minghui63; 20: Gang 46B; 21: Zaogang B; 22: Gufeng B; 23: Jin 23B; 24: Zhenshan 97B; 25: Guangzhan 63S; 26: Peiai 64S. Asterisk indicates the InDel region; Grey arrows indicate the promoter regions of Pi2, Piz-t or Pi9

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