Table 1 Specific primers used in this study
From: Down-Regulation of Cytokinin Oxidase 2 Expression Increases Tiller Number and Improves Rice Yield
Primer name | Sequence (5′ to 3′) | Target gene |
|---|---|---|
Hygromycin-F | GACCTGATGCAGCTCTCGGAG | hptII |
Hygromycin-R | TGCTCCATACAAGCCAACCACG | |
GUS-F | AAAAAACTCGACGGCCTGTGGG | GUS |
GUS-R | GCATCTTCATGACGACCAAAGC | |
Ubiquitin pro.-F | CTGATGCATATACAGAGATGC | a CX3 or CX5 |
Nos ter.-R | TGACAGCTTATCATCGGATC | |
CKX2-F | GTCCACGACGGCGAGCTCAA | OsCKX2 |
CKX2-R | TCCATCTTGGCATCTCTCAG | |
Actin-F | GGTAATGTGTTGGACTCTGG | Actin |
Actin-R | GCAGTGATCTTCCTTGCTCA | |
3′UTR-F | CGGTGACGAGGTGTTCTACAC | OsCKX2 3′ UTR |
3′UTR -R | CCAAGATCTCGTCGTTCTGC | |
rRNA-F | GATAACTCGACGGATCGCACGG | 17S rRNA |
rRNA-R | GCCTGCTGCCTTCCTTGGATGTG | |
OsCKX1-F | GCACCCGTGGCTCAACCTG | CKX1 |
OsCKX1-R | GATGTCGGTGGCCGTCTGG | |
OsCKX3-F | TTTCTTATGCTGATGTGGGTG | CKX3 |
OsCKX3-R | GAACATTGCTAATCTGAGGTCC | |
OsSPL14-F | TGAATTTGACCAAGGAAAAC | SPL14 |
OsSPL14-R | ATCCAACGTAAAGCTTCTGA | |
D53-F | CCAAGCAGTTTGAAGCGAC | D53 |
D53-R | CCGCAAGTTTATCAAAGTCAA | |
OsTB1-F | CAAGAAATCTCGGCGGCTAG | TB1 |
OsTB1-R | CGAATTGGCGTAGACGAC |