Fig. 7
From: Down-Regulation of Cytokinin Oxidase 2 Expression Increases Tiller Number and Improves Rice Yield
Construct of pC1301-CX3 or -CX5 containing a shRNA stem loop for rice transformation. a Diagram of a short hairpin RNA (shRNA) cassette. This cassette comprises inverted repeat sequences (CX3 or CX5) against the target gene (OsCKX2), a spacer fragment (loop) and RNA polymerase III termination sites. b Schematic representation of the transformation constructs (not drawn to scale). shRNA stem loop: 21 nt sense and 21 nt antisense sequences linked by an unpaired loop sequence; Ubiquitin promoter: maize ubiquitin promoter; 35S: CaMV 35S promoter; nos: nopaline synthase terminator; GUS: β-glucuronidase gene; hptII: hygromycin phosphotransferase II gene, LB: left border; RB: right border. c Diagram of self-complementary hairpin siRNA