Fig. 2
From: Down-Regulation of Cytokinin Oxidase 2 Expression Increases Tiller Number and Improves Rice Yield
Southern blot analysis of hptII, CX3 and CX5 in selected T0 primary rice transformants. The T0 primary transformants selected included two independent transgenic lines (CX3-3 and CX3-8) from transformation with shRNA-CX3 (a, b) and three independent transgenic lines (CX5-1, CX5-3 and CX5-5) from transformation with shRNA-CX5 (c, d). WT: untransformed wild type. Genomic DNA was digested with SacI (a, b) or HindIII (c, d) and hybridized with a 32P-labelled probe corresponding to hptII (a, c), CX3 (b), or CX5 (d). The number of reactive bands in each lane represents the transgene copies in each transgenic line