Figure 6

Phenotypes of miR172 -resistant SNB OX plants. (A) Scheme of miR172-resistant SNB (rSNB) construct. Four synonymous mutations (red characters) were introduced into miR172 target sequences at C-terminus of SNB full-length cDNA. Primers tSNB F and R were used. Underlined sequence (tccgga) indicates restriction enzyme site by ACC III. Pact, actin promoter; nos T, nopaline synthase terminator. (B) Expression level of SNB in rSNB OX plants. (C) Phenotypes of rSNB OX plants grown under SD. Bolted panicles are shown by red arrows.