Reduction of orf352 -containing RNA in transgenic plants with PPR782a . (a) Genomic structure around orf352 and orf352-containing transcripts. orf352 is located between rpl5 and nad5 _exon4 _exon5. orf 352 is transcribed as both 4.7-kb and 2.5-kb RNA. Horizontal lines represent both transcripts containing orf352. (b) Northern blot analysis of RNA isolated from leaf blades of a cytoplasmic male sterility (CMS) line (WAA), a fertility restorer line (WAR), and transgenic WAA plants with PPR782a. Staining of ribosomal RNA is shown as a loading control. (c) Expression of the introduced PPR782a and tubulin detected by RT-PCR.