Plasmid construct and PCR analysis of OsAp77::GUS -transgenic lines (T
) of rice. (A) Structure of T-DNA region in the expression construct for rice transformation. Pnos and Tnos, promoter and terminator of nopaline syntase gene, respectively. hpt, hygromycin (hyg) phosphotransferase conferring resistance to hyg; TiaaM, terminator of iaa monooxygenase gene. Arrows indicate the positions of GUS gene-specific primers (Table 1). (B) Gel electrophoresis of PCR-amplified GUS fragment. Lanes 1–3 are derived from line 2A; lanes 4–6, 4A; lanes 7–9, 5A; lanes 10–11, 7A; lane 12, positive control (pBI221 plasmid DNA); lane 13, negative control (non-transgenic line).