Figure 4
From: Introns targeted by plant microRNAs: a possible novel mechanism of gene regulation
Sequencing data-based evidences for the possible novel pathway microRNA (miRNA)— intron —phased small RNAs (sRNAs). (A) Cleavage of the 3rd intron of LOC_OS02G43760.1 by osa-miR1436 resulted in the production of 24-nt phased sRNAs. The region (i.e. 1212 nt to 1356 nt) for phased sRNA production on the full-length gene is shown. The sequence of osa-miR1436 along with its binding site is also shown. The sense strand of the targeted intron was highlighted in blue color, and the antisense strand was in gray. Based on the high-throughput sequencing (HTS) data, the sequences of the phased sRNAs detected by HTS were present in the corresponding boxes, and were numbered accordingly. The accumulation levels of these sRNAs in rdr2, dcl1 and dcl3, and in different Argonautes are shown in the table. Similar illustrations apply to the other figure panels. (B) Cleavage of LOC_OS03G16080.1-6 by osa-miR1436 resulted in the production of 21-nt phased sRNAs. (C) Cleavage of LOC_OS03G16080.1-6 by osa-miR1436 resulted in the production of 21-nt phased sRNAs. (D) Cleavage of LOC_OS06G05250.1-8 by osa-miR1436 resulted in the production of 24-nt phased sRNAs. (E) Cleavage of LOC_OS02G43760.1-3 by osa-miR1436 resulted in the production of 21-nt phased sRNAs. (F) Cleavage of LOC_OS03G16080.1-6 by osa-miR1436 resulted in the production of 21-nt phased sRNAs. Target plots were provided to show the cleavage signals within the miRNA binding sites. For all the target plots, the x axes indicate the positions of the binding sites (marked by blue horizontal lines) on the full-length genes, and the y axes measure the intensity (normalized in RPM, reads per million) of the degradome signals. The figure keys for degradome signatures belonging to different sequencing libraries are shown on the first plot. The intron IDs and the miRNAs are shown on the top of each plot.