Figure 2
From: Diversity and genetics of nitrogen-induced susceptibility to the blast fungus in rice and wheat
Robustness of resistance genes under high nitrogen treatment. Nitrogen (1 N) or no nitrogen (0 N) was added to rice plants one day before inoculation (see Methods). A. Different resistance genes (Pi1, Pi2 and Co39/Pia) were tested using different combinations of rice cultivars and M. oryzae isolates. B. The number of lesions typical of resistance was counted. There were no susceptible lesions under these conditions (white bars: 0 N; black bars: 1 N). The mean of the number of resistant lesions were compared between the 0 N and 1 N conditions and statistical differences are shown by *** (p value < 0.001; Wilcoxon test). C. At the indicated time points after inoculation, the development stage of the fungus was observed and four categories of growth stages were counted 100 interaction sites/condition). This experiment was repeated 3 times and gave similar results. D. The average size of individual fungal colonies was measured inside plant tissues. The mean and sd are shown 50 colonies counted for each condition; white bars: 0 N and black bars: 1 N). Statistical differences are shown by *** (p value < 0.001; Wilcoxon test).