Figure 4
From: RNAi mediated down regulation of myo-inositol-3-phosphate synthase to generate low phytate rice
Expression analysis of transgenic rice plants. (a) RT-PCR analysis of T3 transgenic seeds of MO6-196, as compared to the internal control β tubulin reveals down-regulation in the transcript level of MIPS (b) Expression levels of MIPS as determined by Quantitative real time PCR. The normalized fold expression clearly indicates varied level of silencing, the maximum being 4.59 fold as observed in 196-11-6.