Proteomic analysis of a disease-resistance-enhanced lesion mimic mutant spotted leaf 5 in rice

Rice

Table 1 Identification of proteins differentially expressed between WT and spl5 mutant

Function type	Spot id	Homologous protein	Score	Source	Accession	Coverage (%)	pI	MM (kDa)	Change fold	mRNA level
mRNA splicing	2	Thioredoxin-like protein 4B	71	O. sativa	gi\|125576924	25	6.4	23.6	−3.3	-
Amino-acid metabolism	4	Alanine aminotransferase	187	O. sativa	gi\|115470235	31	8	54.0	−2.3	-
	6	Aspartate aminotransferase	84	O. sativa	gi\|125541475	15	6.5	50.6	2.0	up
	12	Cysteine synthase	86	O. sativa	gi\|115489664	29	5.3	33.9	−3.9	-
	5	S-adenosylmethionine synthetase	93	O. sativa	gi\|100801534	27	6.5	43.0	2.6	up
Photosynthesis	3	Rubisco large subunit	130	O. sativa	gi\|115468792	33	8.5	48.4	−2.7	down
	9	Rubisco activase	74	O. sativa	gi\|1778414	28	5.4	48.1	−2.0	-
	10	Rubisco activase, chloroplast precursor	174	O. sativa	gi\|108864712	44	5.1	36.7	−3.1	-
Glycolysis	8	Glyceraldehyde-3-phosphate dehydrogenase	78	O. sativa	gi\|115459078	24	7.3	36.9	3.1	up
ROS metabolism	11	Glutathione S-transferase 14	88	O. sativa	gi\|46276327	33	6.5	30.8	2.1	up
	13	Ascorbate peroxidase 7	264	O. sativa	gi\|116310282	47	6.9	38.2	8.1	up
Defense-related	14	Chitinase Chia2a	81	O. sativa	gi\|115483206	27	5.4	27.9	3.5	up
Others	1	Retrotransposon Ty3-gypsy subclass	68	O. sativa	gi\|77556153	16	4.7	29.1	3.0	down
	7	Nad-dependent formate dehydrogenase	164	O. sativa	gi\|4760553	40	7.2	41.5	−2.2	-

Spot ID refers to the spot identity as given in Figure 2; Accession, protein accession in GenBank (http://www.ncbi.nlm.nih.gov/); Coverage %, the percentage of sequence coverage; pI, experimental isoelectric points; MM, experimental molecular masses; Change fold, the expression change fold of protein level in spl5 mutant compared to WT; mRNA level, the expression change of mRNA level in spl5 mutant compared to WT.