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Figure 5 | Rice

Figure 5

From: OsRap2.6 transcription factor contributes to rice innate immunity through its interaction with Receptor for Activated Kinase-C 1 (RACK1)

Figure 5

Interaction of OsRap2.6 with OsMAPK6/3 in rice protoplasts. Rice protoplasts were co-transfected with the fluorescent constructs (Vn-osRap2.6 + Vc-OsMAPK6) and (Vn-OsRap2.6 + Vc-OsMAPK3) and examined under fluorescence, bright field and overlay. Known fluorescent proteins mCherry, and OsGenL-CFP (nuclear marker) were used as markers of localizations. Conditions for microscopy and data analysis were identical to those outlined in the legend to Figure 1. (A) Interaction between OsRap2.6 and OsMAPK6 compared with the mCherry construct. (B) Quantitative analysis of BiFC positive cells from (A) (OsRap2.6 + OsMAPK6). (C) Interaction between OsRap2.6 and OsMAPK3 compared with the mCherry. (D) Quantitative analysis of BiFC positive cells from (C) (OsRap2.6 + OsMAPK3). (E) Frequency (%) of interactions between OsRap2.6 and OsMAPKs in cells.

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