Fig. 5

Validation of responsive unannotated transcripts. The expression of OsIPS1 and several unannotated transcripts in response to –P and ++P was validated by qRT-PCR analysis. OsIPS1 is a –P upregulation index gene with statistical significance in the roots (upper) and shoots (lower) as determined by G test. Transcript expression levels were normalized using an internal control (Ubiqutin1) and plotted relative to the expression on day 10 during –P/++P and at 1 day after recovery from stress (Re1). The bars represent the mean ± SE level of transcripts from three experiments. The asterisk indicates the responsive point based on G test (FDR, <0.01). The primers used in qRT-PCR analysis for unannotated transcripts are described in Supplementary Table S5.