Table 1 Primer pairs for real-time RT-PCR
From: Characterization of OsSUT2 Expression and Regulation in Germinating Embryos of Rice Seeds
Gene | Accession number | Primer pair | Amplicon size (bp) |
|---|---|---|---|
(F: forward primer; R: reverse primer) | |||
OsSUT1 | D87819 | F: 5′-CTGTGATTTTCCTGTCCCTG-3′ | 136 |
R: 5′-AACACTGCTAGTGGACCAGT-3′ | |||
OsSUT2 | AB091672 | F: 5′-AGGAGGAGAGGTCACCGATAA-3′ | 240 |
R: 5′-CCAACATCCAATGTACAACAGCA-3′ | |||
OsSUT3 | AB071809 | F: 5′-GCCCAAGGTCTCCGTCC-3′ | 137 |
R: 5′-TGCTATAGTACCCGCTCTAA-3′ | |||
OsSUT4 | AB091673 | F: 5′-TTTGGCTGAGCAGAACACCA-3′ | 249 |
R: 5′-ATGTCATTCGGGCAGAGCTT-3′ | |||
OsSUT5 | AB091674 | F: 5′-CTAGTGCGAAACTCCATCAAA-3′ | 249 |
R: 5′-AAAATATTTGGGTTTCCTGAGAT-3′ | |||
Ubi | D12629 | F: 5′-CGCAAGTACAACCAGGACAA-3′ | 101 |
R: 5′-TGGTTGCTGTGACCACACTT-3′ |