Fig. 4

OsSUT2 expression and glucose content in embryonic cells from light- and dark-grown seedlings. Rice seeds were germinated in the dark for 3 days. Some germinated seeds were moved to a phytotron with natural daylight for 2 days (labeled 3-day D/2-day L), and some germinated seeds were kept in the dark (labeled 5-day D) for growth. The seedlings were collected at 1 and 5 DAI, and the embryos were isolated for OsSUT2 expression analysis (a) and to measure glucose content (b). c The OsSUT2 expression in germinating isolated embryos was analyzed. The embryos were dissected from dry seeds and dark-grown in MS medium. OsSUT2 expression was detected after 1, 3, and 5 days of culture. The data are presented as mean ± SD.