Fig. 8
From: Folate Polyglutamylation is Required for Rice Seed Development
Transcript profiling of folate-related genes during seed developmental stages was analysed by RT-qPCR in Dongjin wild-type and fpgs03g mutant. Per one determination, 40 fresh seeds from each stage from different five plants were pooled. Only dominant folate-related genes was considered which are a GTPCHI LOC_Os04g56710 (GTP), bADC synthase LOC_Os06g48620 (ADC syn), cHPPK/DHPS LOC_Os07g42632 (HPPK/DHPS), dFPGS LOC_Os10g35940 (FPGS_10g) and eGGH LOC_Os05g44130 (GGH). Head is heading stage of panicles. Anthesis is the flowering stage which is the first day of the florets open. Milk is the white milky liquid seed stage. Dough is the green hard dough seed stage. Dry is the brown mature seed stage. Each stage was profiled in triplicate. Asterisks represent the significant difference (P < 0.05) of transcript abundance compared between knockout and Dongjin wild type within tissues.