a NA and DMA concentration in T2 seeds obtained from plants cultured in small pots fertilized with nutrient solution. White bars, NA; gray bars, DMA. Distinct letters above the bars indicate significant differences (p < 0.05). b–e Scatter diagram between NA or DMA concentration in T2 seeds and Fe or Zn concentration in polished T2 seeds for each plant cultured in small pots fertilized with nutrient solution. b NA and Fe concentrations. c NA and Zn concentrations. d DMA and Fe concentrations. e DMA and Zn concentrations. White circle, NT lines; black square, 35S lines; white square, AN1 lines; black triangle, AN3 lines.