Table 1 Expression Levels and Disease Rating of Transgenic Lines
From: Transgene Stacking and Coordinated Expression of Plant Defensins Confer Fungal Resistance in Rice
Constructs | Transgenic line | Expression level (µg/mg of total protein) | Disease rating | ||
|---|---|---|---|---|---|
Dm-AMP1 | Rs-AFP2 | % DLA (M. oryzae) | % infection (R. solani) | ||
pUbI-DR-T | |||||
Untransformed | 0.00 | 0.00 | 69.68 | 66.33 | |
DR-T-11 | 7.40 | 7.20 | 14.38 | 25.00 | |
DR-T-6 | 8.15 | 8.03 | 13.12 | 20.83 | |
DR-T-4 | 8.60 | 8.40 | 13.00 | 14.58 | |
DR-T-22 | 9.15 | 8.70 | 9.89 | 14.38 | |
Average | 8.33 | 8.08 | 12.59 | 18.69 | |
pUb1-D-T | |||||
Untransformed | 0.00 | – | 69.68 | 66.33 | |
Dm-T-16 | 4.32 | – | 18.35 | 28.00 | |
Dm-T-21 | 5.04 | – | 10.74 | 18.67 | |
Dm-T-14 | 4.79 | – | 14.74 | 20.70 | |
Dm-T-32 | 5.69 | – | 10.12 | 18.58 | |
Average | 4.96 | – | 13.48 | 21.49 | |
pUb1-R-T | |||||
Untransformed | – | 0.00 | 69.68 | 66.33 | |
Rs-T-31 | – | 4.50 | 25.64 | 48.85 | |
Rs-T-26 | – | 4.80 | 15.17 | 43.08 | |
Rs-T-24 | – | 5.20 | 11.73 | 38.23 | |
Rs-T-42 | – | 5.29 | 11.01 | 36.38 | |
Average | – | 4.95 | 15.89 | 41.64 | |
SE | 0.18 | 0.10 | 0.66 | 1.11 | |
5%LSD | 0.53 | 0.19 | 1.87 | 3.17 | |