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Table 1 Expression Levels and Disease Rating of Transgenic Lines

From: Transgene Stacking and Coordinated Expression of Plant Defensins Confer Fungal Resistance in Rice

Constructs Transgenic line Expression level (µg/mg of total protein) Disease rating
Dm-AMP1 Rs-AFP2 % DLA (M. oryzae) % infection (R. solani)
pUbI-DR-T
  Untransformed 0.00 0.00 69.68 66.33
  DR-T-11 7.40 7.20 14.38 25.00
  DR-T-6 8.15 8.03 13.12 20.83
  DR-T-4 8.60 8.40 13.00 14.58
  DR-T-22 9.15 8.70 9.89 14.38
  Average 8.33 8.08 12.59 18.69
pUb1-D-T
  Untransformed 0.00 69.68 66.33
  Dm-T-16 4.32 18.35 28.00
  Dm-T-21 5.04 10.74 18.67
  Dm-T-14 4.79 14.74 20.70
  Dm-T-32 5.69 10.12 18.58
  Average 4.96 13.48 21.49
pUb1-R-T
  Untransformed 0.00 69.68 66.33
  Rs-T-31 4.50 25.64 48.85
  Rs-T-26 4.80 15.17 43.08
  Rs-T-24 5.20 11.73 38.23
  Rs-T-42 5.29 11.01 36.38
  Average 4.95 15.89 41.64
SE   0.18 0.10 0.66 1.11
5%LSD   0.53 0.19 1.87 3.17
  1. Levels of Dm-AMP1 and/or Rs-AFP2 in transgenic lines, Blast and sheath blight infection assay results (% DLA and % infection) were compared by one-way analysis of variance (ANOVA). Means were compared using LSD5%. Design of experiment was completely randomized block design (CRD)