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Table 1 Differential Display Proteome Analysis of Abiotic Stress Treatments in Rice

From: Research on the Rice Proteome: The Contribution of Proteomics Technology in the Creation of Abiotic Stress-Tolerant Plants

Identified proteins
Genotype Growth stage, tissue Treatment Stress duration Proteomic approach T value C value Major result References
Drought stress
Nipponbare, Zhonghua (T) Leaf sheath Progressive water 2 to 6 days 2D-PAGE/sequencing 12 12 Up-regulation of actin depolymerizing factor [4]
CT9993 (T), IR62266 Leaf Progressive water (23 days)/recovery (10 days)   2D-PAGE/MALDI-TOF, Q-TOF MS 42 16 Stressed leaf WP = −2.4 Mpa/up-regulation of S-like RNase, actin depolymerizing factor and down-regulation of isoflavone reductase-like protein [49]
Salt stress
Nipponbare Root (20 days seedling) 150 mM NaCl 0, 10, 24 h 2D-PAGE, Pro-Q diamond/MALDI-TOF MS 214 28 Pro-Q diamond was used to study phosphoproteome. About 214 proteins were detected as phosphoproteins [8]
IR651 (T) Root (8 weeks) 100 mM NaCl 2 weeks 2D-PAGE/MALDI-TOF MS 164 24 Plasma membrane-associated protein was used. 1,4-Benzoquinone reductase, remorin and hypersensitive induced response protein were identified as salt responsive proteins [42]
Nipponbare, Pokkali (T) Leaf sheath 50, 100, 150 mM NaCl 6 to 48 h 2D-PAGE/Sequencing 8 5 IR36 was analyzed/coordinated response of different parts of the rice to NaCl [1]
Nipponbare Root 150 mM NaCl 24, 48, 72 h 2D-PAGE/MALDI-TOF MS 54 12 Several novel salt responsive proteins like α-NAC, COX6b-1, UGP [57]
Nipponbare Leaf 130 mM NaCl 4 days 2D-PAGE/LC MS/MS 55 33 Hydroponic culture system is best suited for proteomics of salt stress in rice seedling [26]
IR4630 (M) Leaf lamina 50 mM NaCl 1, 7 days 2D-PAGE/MALDI TOF-TOF MS 32 11 More responsive proteins in long- compare to short-term stress/defining threshold value to screen significant changes [44]
IR651 (T) Young panicle 70 mM NaCl 10 days 2D-PAGE/MALDI TOF-TOF MS 13 13 Constitutive high expression and/or up-regulation of ROS scavenging enzymes [12]
Cold stress
Doongara (S) Anther/trinucleate stage 12°C 4 days 2D-PAGE/MALDI-TOF MS 70 18 Chilling temperature stress at the young microspore stage enhances and induces partial degradation of proteins [22]
NA Leaf 15°C 24 h 2D-PAGE/MALDI-TOF, Q-TOF MS 60 41 43% of identified proteins were located in chloroplast. So chloroplast proteome is virtually subjective to cold stress [9]
Doongara (S), HSC55 (T) Anther/young microspore 12°C 1, 2, 4 days 2D-PAGE/MALDI-TOF, Q-TOF MS, sequencing 37 16 The specific expression of responsive proteins tolerant compare to susceptible cultivar [23]
Nipponbare Leaf 6°C for 6, 24 h/24 h recovery 2D-PAGE/MALDITOF-TOF MS 96 85 mRNA level of stress responsive genes was not correlated well with the protein level [58]
Nipponbare, Kitaibuki (T), IR36, Er Jiu-Qing, Jamura, Kele (S) Leaf 1, 5, 15°C 6 h 32P-labeling, 2D-PAGE/sequencing 50 10 32P-labeling was used to study phosphoproteome. Out of 10 cold responsible proteins, RuBisCO large-subunit was identified [30]
Nipponbare, Kitaake (T), Basmati 370 (S), transgenic (T) Leaf sheath 4°C 24 h 2D-PAGE/MALDI-TOF MS, sequencing 456 6 As transgenic rice, over-expression of CDPK13 and CRTintP1 was used. These two genes were binding to calreticulin [34]
Nipponbare Basal part of leaf sheath 4°C 48 h 2D-PAGE, lectin blot/MALDI-TOF MS 250 22 To analyze the glycosylation during cold stress, lectin blot was used [33]
Nipponbare Leaf blades, leaf sheath, root 4°C 48 h 2D-PAGE, RuBisCO removing/LC MS/MS 400 39 Antibody-affinity column was prepared to trap RuBisCO large subunit, and 4 proteins were newly detected after cold stress [20]
Dondjin Leaf 5, 10°C 24, 72 h 2D-PAGE, RuBisCO removing/MALDI-TOF MS, ESI MS/MS   12 Fractionation by PEG was used to trap RuBisCO large subunit [37]
Dondjin Root 10°C 24, 72 h 2D-PAGE/MALDI-TOF MS, ESI MS/MS 600 27 Acetyl transferase, phosphogluconate, NADP-specific isocitrate dehydrogenase, fructokinase, PrMC3, alpha-soluble N-ethylmaleinmide-sensitive factor attached protein and glyoxalase were found as cold responsive proteins [38]
Ozone stress
Nipponbare Leaf 0.2 ppm Ozone 24, 48, 72 h 2D-PAGE/Sequencing 52 37 Reduction in RuBisCO and induction of various defense/stress related proteins [2]
Koshihikari Leaf 5, 40, 80, 120 ppb 9 days 2D-PAGE/sequencing, MALDI-TOF MS 300 20 PR5, PR10 and RSPR10 were significantly induced after 2 days exposure [15]
Other stresses
Hwayeong Germinating seed 0.2 to 1.5 mM Copper 4 days 2D-PAGE/MALDI TOF MS 25 25 The majority of these proteins were antioxidants or stress-related regulatory proteins [3]
Nipponbare, Zhonghua (T) Basal part of leaf sheath 0 to 1,000 mM mannitol 0 to 120 h 2D-PAGE/sequencing, MALDI-TOF MS 327 15 Heat shock protein and dnaK-type molecular chaperone were induced under osmotic, cold, salt, drought and ABA were reduced, whereas 26S proteasome regulatory subunit was found to be responsive only to osmotic stress [59]
Nipponbare Leaf sheath Water (wound) 0, 12, 24, 48 h 2D-PAGE/Sequencing, MALDI-TOF MS 29 12 Identification of wound responsive proteins including Bowman-Birk trypsin inhibitor, receptor-like protein kinase, and calmodulin-related protein [52]
Multiple stresses
Nipponbare leaf sheath, leaf blade, root 50 mM NaCl (salt), 12°C (cold) 24 h 32P-labeling, 2D-PAGE/MALDI-TO, Q-TOF MS 4, 4 2, 3 Regulation of phosphorylation was studied [24]
Nipponbare Root 100 mM NaCl (salt), drought, 100 μM ABA 15 h 2D-PAGE/sequencing 300 13 RSPR10 was a novel rice PR10 protein, which was rapidly induced in roots by salt and drought stresses [19]
  1. S Sensitive, T tolerant, T total, C changed by stress