Validation of microarray data by quantitative RT-PCR. a The expression of 14 genes selected from Table 1 was analyzed through quantitative RT-PCR. The results of three independent experiments for both BTx623 and Rio are shown. The quantification of the mRNA abundance for each gene is presented as relative fold expression. b qRT-PCR comparing the expression of saposin type B and fasciclin-like protein FLA15 in BTx623 and two sweet sorghum lines Della and Dale.