Fig. 2

Illustration of production of publicly acceptable transgene-free climate resilient rice varieties using gene editing tools. (A) Different biotic and abiotic stresses. (B) Diseased or under stress (A/biotic) plants having susceptibility (Su) and sensitivity (Se) genes. (C) Various crop improvement breeding interventions. (D) Gene-edited plant/s (T0) developed using GE tools e.g., CRISPR-Cas-based GE system. At this stage plant/s could have heterozygous or homozygous mutation, hence screening of homozygous plants is required. (E) Final genetically stable homozygous transgene-free desired rice plant/s that can be released commercially as a potential new variety or can be used in rice breeding programs