Fig. 3
From: OsRLCK 57, OsRLCK107 and OsRLCK118 Positively Regulate Chitin- and PGN-Induced Immunity in Rice
OsRLCK107 interacts with OsCERK1. a OsRLCK107 interacts with OsCERK1 by BiFC. OsRLCKs-YN and OsCERK1-YC were co-expressed in rice protoplasts, the reconstituted YFP fluorescence was visualized using confocal microscope. Chl indicated the chlorophyll autofluorescence. Positive control: co-expression of bZIP63-YN and bZIP63-YC, negative control: co-expression of OsRLCKs-YN and blank-YC. Bar = 10 μm. b The interaction between OsRLCK107 with OsCERK1 is regulated by chitin and PGN. Protoplasts expressing OsRLCK107 with a C-terminal 3 × FLAG epitope tag were treated with 150 μg/mL chitin、PGN or sterile water (for mock) for 10 min. After treatment, protein was prepared for immunoprecipitation with anti-FLAG GEL (Sigma). The presence of OsCERK1 in the complex was analyzed by OsCERK1 antibody. Protein exacted from wide type (WT) protoplasts was used as negative control. Three biological replicates were performed and similar results were obtained