OsRLCK 57, OsRLCK107 and OsRLCK118 Positively Regulate Chitin- and PGN-Induced Immunity in Rice
- Zhangqun Li†1,
- Ying Ao†1,
- Dongru Feng†1,
- Jun Liu1,
- Jinfa Wang1,
- Hong-Bin Wang1 and
- Bing Liu1Email authorView ORCID ID profile
© The Author(s). 2017
Received: 17 August 2016
Accepted: 14 February 2017
Published: 21 February 2017
The receptor-like cytoplasmic kinase, OsRLCK176 has been reported to participate in both chitin- and PGN-induced immunity in rice. Here, we further researched the function of the homologous proteins, OsRLCK57, OsRLCK107 and OsRLCK118, in chitin- and PGN immunity in rice.
Silencing of OsRLCK57,OsRLCK107 and OsRLCK118 suppressed chitin- and PGN-induced immunity responses, including reactive oxygen species generation, defense gene expression. Furthermore, OsRLCK107 could interact with OsCERK1 in a MAMP induced way, which suggested a possible physiological relevance of OsRLCKs107 to OsCERK1 pathway.
OsRLCK57, OsRLCK107 and OsRLCK118, positively regulate chitin- and PGN- induced responses in rice, similar to that observed in OsRLCK176.
KeywordsReceptor-Like Cytoplasmic Kinases Microbe-Associated Molecular Pattern Pattern Triggered Immunity Rice
PTI requires cytoplasmic members to link PRR activation with downstream signaling and RLCKs have emerged as key roles in the mechanism. In this research, we identified three members of RLCK group VIIa, OsRLCK57, OsRLCK107 and OsRLCK118, play roles in chitin and PGN immunity in rice. These results presented here will provide novel insight into the downstream signaling regulation of the chitin and PGN immune responses in rice.
Microbe-Associated Molecular Pattern
Pattern Recognition Receptor
Pattern Triggered Immunity
Receptor-Like Cytoplasmic Kinase
This research was supported by the National Natural Science Foundation of China (No. 31100874 and No. 31425003), the Natural Science Foundation of Guangdong Province, PR China (No.S2011040001868), the Fundamental Research Funds for the Central Universities (12lgpy33 and 14lgzd04), and the project of Science and Technology New Star in Zhujiang City (2012–78).
ZL, YA and DF performed research. JL analyzed data. BL, HBW and JW designed the research. All authors read and approved the final manuscript.
The authors declare that no competing interests exist.
Gene sequence data referred to in this article can be found in the GenBank/EMBL databases under the following accession numbers: OsRLCK107(Os03g0274800), OsRLCK57(Os01g0973500), OsRLCK118(Os03g0821900), OsRLCK176(Os05g0110900), OsRLCK185(Os05g0372100), OsCERK1(Os08g0538300), OsPR5(Os01g0122000), OsPR10 (Os03g0300400), PAL (Os02g0627100), PBZ1 (Os12g0555500).
[BIK1: AT2G39660, NAK: AT5G02290, PBL1: AT3G55450, PBL27: At5G18610, APK1A: AT1G07570, APK1B: AT2G28930, PBS1: AT5G13160, NAK: AT5G02290, PBL1: AT3G55450].
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