Fig. 1

Characterization of homologous OsRLCK57,OsRLCK107 and OsRLCK118. a OsRLCK57, OsRLCK107, OsRLCK118 is highly homologous with OsRLCK176 and BIK1. The phylogenetic tree was generated by MEGA4. Full length amino acid sequences were selected to generate the bootstrap neighbor-joining phylogenetic tree. Bootstrap probabilities were obtained from 1000 replicates. Scale bar is indicated. b OsRLCK57, OsRLCK107 and OsRLCK118 is plasma membrane localized protein. Subcellular localization of OsRLCK57, OsRLCK107 and OsRLCK118 was visualized using confocal microscope after transiently transformed OsRLCK57-GFP, OsRLCK107-GFP and OsRLCK118-GFP into rice protoplasts. Chl indicated the chlorophyll autofluorescence. Three biological replicates were performed, similar results were obtained. Bar = 10 μm. c Expression identification of RLCKs in different RNAi lines. The relative expression levels OsRLCK57, OsRLCK107 and OsRLCK118 were examined by quantitative RT-PCR in OsRLCK57, OsRLCK107 and OsRLCK118 RNAi lines, respectively. The expression of OsRLCK176 and OsRLCK185 was also examined in these RNAi lines to exclude its effects. Empty vector (1301) transgenic callus cells were used as control. 57i-1 and 57i-14: RNAi lines of OsRLCK57. 107i-5 and 107i-6: RNAi lines of OsRLCK107. 118i-1 and 118i-2: RNAi lines of OsRLCK118. The data represents the mean ± standard deviation (SD) from three biological replicates