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Fig. 1 | Rice

Fig. 1

From: Genome-Wide Effects on Gene Expression Between Parental and Filial Generations of Trisomy 11 and 12 of Rice

Fig. 1

Fluorescence in situ hybridization (FISH) identification of aneuploid and diploid on mitotic metaphase chromosomes in rice. a and b Bar-code oligo-FISH analysis of parental primary trisomy 11 and 12 (named as T11-P and T12-P) using FAM-green and digoxigenin-red labeled probes on somatic cell chromosomes in mitotic metaphase, respectively. c and d Bar-code oligo-FISH analysis of filial primary trisomy 11 and 12 (named as T11-F and T12-F) using FAM-green and digoxigenin-red labeled probes on somatic cell chromosomes in mitotic metaphase, respectively. e and f FISH analysis of normal strain of progeny of parental primary trisomy 11 and 12 (named as T11-FN and T12-FN) using FAM-green and digoxigenin-red labeled probes on mitotic metaphase chromosomes, respectively. g FISH analysis Zhongxian 3037 using FAM-green and digoxigenin-red labeled probes on mitotic metaphase chromosomes. h–n The seedling phenotype corresponding to (a–g). Bars = 5 cm. The yellow arrow points to the extra chromosomes. Chromosomes were counterstained with 4’,6-diamidino-2-phenylindole. Bars = 5 μm

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