Fig. 5
From: Ubiquitin-Conjugating Enzyme OsUBC11 Affects the Development of Roots via Auxin Pathway
Determination of ubiquitin activity of OsUBC11. The recombinant protein purified result of OsUBC11-pET32b (a) and OsUBC11-Mu-pET32b (b) U1, U2, U3 represent elution times in OsUBC11, M1, M2, M3 represent elution times in OsUBC11 mutant protein. c, d Reaction of ubiquitin cascade of OsUBC11 or OsUBC11 mutant. Ubiquitinated proteins were detected by immunoblotting with anti-His (c) and anti-Ub (d). e–f Confirm the polyubiquitin-chain way of OsUBC11. Ubiquitinated proteins were detected by immunoblotting with anti-His (E) and anti-Ub (F)