Fig. 2
From: Ubiquitin-Conjugating Enzyme OsUBC11 Affects the Development of Roots via Auxin Pathway
Identification of the T-DNA insert situation. a Schematic of the genomic region flanking the T-DNA insertion site in R164. 62230, 62244, 62260 and 62290 represent LOC_Os01g62230, LOC_Os01g62244, LOC_Os01g62260 and LOC_Os-01g62290 respectively; four arrow represent the four tandem copies of the CaMV35S enhancer near the T-DNA right border. LB, T-DNA left border. RB, T-DNA right border. The black arrows represent the P1, P2, and P3 primers used in find insert site. b Semiquantitative analysis of the insert site nearby genes, OsUBI5 were amplified as control. c qRT-PCR analysis of the expression of OsUBC11 between R164 mutant and WT Zhonghua11,OsUBI5 transcripts were amplified as control. Red spot represented a duplicate value. Two asterisks (**P < 0.01) represent significant differences between the WT and mutants. d The OsUBC11 protein domain. e Cladogram of OsUBC11