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Fig.4 | Rice

Fig.4

From: Analysis of the Genetic Stability of Insect and Herbicide Resistance Genes in Transgenic Rice Lines: A Laboratory and Field Experiment

Fig.4

Southern blot analysis of the total genomic DNA of BC4F8 and BC4F9 transgenic plants. Southern blot of a CHT025(1C), b CHT025(2A), c CH891(1C), d CH891(2A), e CH871(1C), and f CH121(1C) in BC4F8 and BC4F9 transgenic plants. The DNA samples were digested with SmaI and hybridized with the prepared radioactive probe (SmaI restriction sites are absent within the T-DNA region). The size of the probe was 6.8 and 3.0 kb for SmaI and was a fragment of the BAR gene. M: DNA marker; 1 and 6: negative control; 2 and 7: positive control; 3 and 8: BC4F8 and BC4F9 transgenic plants, respectively; 4 and 9: PCR product. 1–4: southern blot analysis BC4F8 in 2020; 1–4: southern blot analysis BC4F9 in 2021

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