Fig. 3
Southern blot analysis of the total genomic DNA of BC4F8 and BC4F9 transgenic plants. Southern blot of a CHT025(1C), b CHT025(2A), c CH891(1C), d CH891(2A), e CH871(1C), and f CH121(1C) in BC4F8 and BC4F9 transgenic plants. The DNA samples were digested with NcoI and HindIII and hybridized with the prepared radioactive probe (NcoI and HindIII restriction sites are absent within the T-DNA region). The size of the probe was 4.3 and 5.5 kb for NcoI and 4.1 kb for HindIII and was a fragment of the CRY1C gene. The DNA samples were digested with BamHI and EcoRI and hybridized with the prepared radioactive probe (BamHI and EcoRI restriction sites are absent within the T-DNA region). The size of the probe was 2.5 kb for BamHI and 3.5 kb for EcoRI and was a fragment of the CRY2A gene. M: DNA marker; 1 and 4: negative control; 2 and 5: positive control; 3 and 6: BC4F8 and BC4F9 transgenic plants, respectively; C: PCR product