Marker-Assisted Introgression of the Salinity Tolerance Locus Saltol in Temperate Japonica Rice

Rice

Table 4 The results and statistics of phenotypic evaluations of BC₃F₄ introgression lines and the recurrent parent Onice (A) and BC₃F₄ introgression lines and the recurrent parent Vialone Nano (B), tested in two experimental sites (Malalbergo and Vercelli). ANOVA and Dunnett’s test significant for p(F) < 0.05

Variable	Unit	Site	Total avg	ANOVA	Dunnett's test			O1/all lines*100 (index)^(b)
Variable	Unit	Site	Total avg	p(F)	Recurrent parent	O1 line^(a)	LSD	O1/all lines*100 (index)^(b)
(A)
Yield	Paddy 13% t ha⁻¹	Malalbergo	6.21	0.075	6.46	6.76	1.18	109.6
Yield	Paddy 13% t ha⁻¹	Vercelli	10.1	0.022*	10.6	10.6	2.94	104.2
Plant height	cm	Malalbergo	84.1	< 0.01**	74.3	74.3	7.97	86.9
Plant height	cm	Vercelli	100.2	< 0.01**	94.5	92.7	10.7	91.8
Panicle lenght	cm	Malalbergo	13.8	0.146	13.5	13.2	3.57	95.0
Panicle lenght	cm	Vercelli	16.6	0.030*	16	16.1	3.36	97.0
Seed number	Number per panicle	Malalbergo	71.2	0.766	72.2	69.0	20.0	97.2
Seed number	Number per panicle	Vercelli	92.8	< 0.01**	120.2	95.3	46.2	107
Flowering	Days from 1st July	Malalbergo	45.6	0.272	48.5	41.5	7.56	91.8
Flowering	Days from 1st July	Vercelli	46.2	< 0.01**	43.0	47.3	4.55	101.3
Sterility	Scale	Malalbergo	1.19	0.707	1.0	1.0	1.43	82.6
Sterility	Scale	Vercelli	1.13	0.410	1.0	1.0	1.09	87.7

Variable	Unit	Site	Total avg	ANOVA	Dunnett's test				VN1/all lines*100 (index)^(d)	VN4/all lines*100 (index)^(e)
Variable	Unit	Site	Total avg	p(F)	Recurrent parent	VN1 line^(c)	VN4 line^(c)	LSD	VN1/all lines*100 (index)^(d)	VN4/all lines*100 (index)^(e)
(B)
Yield	Paddy 13% t ha⁻¹	Malalbergo	6.26	0.09	6.42	6.08	6.35	1.82	97.1	101.4
Yield	Paddy 13% t ha⁻¹	Vercelli	9.34	0.17	N.A.⁽^f)	8.51	9.33	−	93.3	102.3
Plant height	cm	Malalbergo	121	0.046*	120	113	114	23.8	93.2	94.1
Plant height	cm	Vercelli	132	0.21	132	136	129	13.6	102.9	97.6
Panicle lenght	cm	Malalbergo	19.7	0.29	21.2	19.5	19.8	3.21	100.5	102.1
Panicle lenght	cm	Vercelli	19.2	0.53	18.8	20.1	19.2	2.99	104.1	99.5
Seed number	Number per panicle	Malalbergo	120	0.87	128	110	117	51.2	92.4	98.3
Seed number	Number per panicle	Vercelli	120	0.23	104	123	138	47.5	101.7	114.0
Flowering	Days from 1st July	Malalbergo	48.5	0.018*	48.5	49.0	50.5	6.38	101.0	104.1
Flowering	Days from 1st July	Vercelli	52.7	< 0.01**	50.0	55.7	54.0	2.97	104.3	101.1
Sterility	Scale	Malalbergo	1.1	0.5	1.0	1.0	1.0	0.89	92.6	92.6
Sterility	Scale	Vercelli	1.2	0.190	1.0	0.7	1.0	1.56	54.9	82.0

Asterisks indicate statistically significant differences (*p(F) < 0.05; **p(F) < 0.01)
^(a)O1 introgressed line selected for the hydroponic assessment
^(b)Average of O1 line/average of all Onice lines*100
^(c)VN1 and VN4 introgressed lines selected for the hydroponic assessment
^(d)Average of VN1 line/average of all Vialone Nano lines*100
^(e)Average of VN4 line/average of all Vialone Nano lines*100
^(f)N.A. not available