Fig. 3

OsDPE2 affects vegetative plant development of rice via DPE2 enzyme activity, and DPE2 enzyme activity fluctuates rhythmically due to diurnal alternations. a–c Growth rate analysis of the plants under long day (LD, 16 h light/8 h dark) and short day (SD, 8 light/16 dark) conditions. a Images of wild-type and osdpe2#01 seedlings under LD and SD. Number of tillers (b) and plant height (c) of wild-type and osdpe2#01 seedlings under LD and SD conditions. Data are expressed as mean ± SD (n = 10 biologically independent samples). Bars represent mean ± standard deviation; Tukey's multiple comparisons test. Different letters represent statistically significant differences between data groups. d DPE2 enzyme activity and e OD values in ML, YL, ST, RO, P1, P2, P3, and P4. Three samples of ML, YL, ST and RO were mixed to form a biological replicate, and each data group contained three biological replicates. One biological replicate contained ten young panicle tissues. Bars represent mean ± SE (one-way ANOVA). f The 48-h rhythmic OsDPE2 expression analysis of the WT plants under LD and SD conditions. g The 48-h rhythmic in-gel DPE2 enzyme activity assay and h OD values of the DPE2 enzyme activity assay of WT under LD and SD conditions. The arrow indicates DPE2 enzyme activity